water sampling procedure for microbiological analysis

Carry the sampling containers to the sampling point as per the sampling schedule. Details of the type of sample containers and preservatives to be used are also included. Sampling Instructions This method of sample collection is acceptable for EPA analytical methods 1622 and 1623 when sending water samples in to the laboratory without filtering in the field. OBJECTIVE:To lay down a procedure for microbiological monitoring of raw water and purified water. <> 3. Water system sanitization methods require assessment. This Standard Operating Procedure is applicable to the Microbiology Department. Then remove the sterile strip from the bottle. Close the valve and bring the samples to the laboratory and make an entry in the purified water inward register. Alert levels are always lower than Action levels. Approximately add 15-20 ml of R2A / Plate count Agar into each Petri dishes. These procedures should be utilized by drinking water service providers. Use sodium hypochlorite solution (1g/L free chlorine) or ethanol/isopropanol 70% v/v. After completion of the test, record the results in the datasheet and also enter the results in the respective software/module. Analyze the sample as quickly as possible on arrival at the laboratory. Analyze the TOC sample as quickly as possible on arrival at the laboratory. 2 Content • Introduction – What does the future hold for microbiological water AQC? SCOPE:This SOP shall provide the procedure for sampling and testing of raw water and purified water from all the user points and all the points across the critical functions in the water purification process. Annexure-1: List of Sampling Points of Purified Water. 1. 4. 1 minute. Prepare the sampling schedule (Tentative) by the first working day of every New Year. If you are sampling more than one location you need a separate sample bottle for each location. Head of the Department / Designee of respective areas & QA shall be responsible for reviewing the SOPs. ^���\�L �&�BϘ�B���X�d&ǣ=�K�}. Close the bottle with a cap and wrap the neck with aluminum foil and Sterilize the bottle at 121 ºC (15 lbs.) 3. %PDF-1.5 Phase 2 follows the determination of data validity and investigates the water system and all potentially affected lots of products. 4.1.16 Again collect the swab sample and repeat procedure following Point 4.1.1 to 4.1.13. Collect the purified water sample (refer table 1) without rinsing and close it with stopper immediately. The changes may occur in the bacterial content of water on storage can be reduced to a minimum by ensuring that samples are not exposed to light and are kept cool. Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. <>>> Head QA shall be responsible for the approval of SOP. Analyst error should be rare. The precautions taken to avoid contamination must be such that they do not affect any microorganisms that are to be revealed in the test (water analysis). PROCEDURE FOR MICROBIOLOGICAL MONITORING OF WATER. Analyse the water samples for Microbiological analysis as per specifications. Sampling … Where potable (drinking water) water is stored, BS8558:2015 sets out a regime where 6 monthly microbiological activity is measured. Before analysis, Register/log the sample, and generate/issue the worksheet, or the template. In particular, the spread of enteric viruses, e.g. If any chemical parameter exceeds the limit, immediately inform the same QA, Production, and Engineering Department. HOW TO COLLECT A WATER SAMPLE FOR MICROBIOLOGY EXAMINATION Created: March 26, 2015 Page 1 of 2 *Conditions Apply 1. Write the sampling details or affix the label on the bottle as per Annexure-3. RESPONSIBILITY Quality Control Executive / Officer ACCOUNTABILITY Quality Assurance Head. Refer Annexure-2 for preparing the Sampling Schedule. Wrap the neck with aluminum foil to avoid any contamination during transportation. Sampling personnel shall wear clean smocks or gowns and sterile gloves prior to collecting any sample (As per area requirements). Moreover, there are many types of pathogen and each requires a unique microbiological isolation technique. 4 0 obj Sanitize the hands with 70 % v/v IPA. Domestic water systems are supplied with water fit to drink, so ACoP L8 advices that regular microbiological sampling is not required. Water quality — Sampling for microbiological analysis. <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> 4.13 Carry out sampling step 4.14 and 4.19 as rapidly as possible to avoid exposure of the sample to the external environment. After completion of the analytical report, checked and approved by a designated person and after that generate the COA (if required). Wear gloves when collecting samples. Avoid unpredictable changes in the microbial population ii. Step Two Wash Your Hands prior to taking the sample. Collect the 100ml of water sample for self analysis and 500ml of water for outside lab for microbiological analysis … PROCEDURE: Sampling of water Sampling of water … Wear gloves and nose mask before sampling. Noroviruses, are repeatedly related to poor water quality (… Sampling apparatus and containers shall be clean, sterile, and/or endotoxin-free as per test requirements. Water Sampling Procedure (with reference to ISO 5667 Part 5) (A) Assessment of Sampling Location Please take note of the conditions of the environment, sampling taps and pipes at the sampling location. It is important to note that you don’t have to exceed specification in order to submit a result for identification, it’s a best practice to document and select and identify representative flora from the system. Sampling water for subsequent analysis is often considered to be somewhat easier than sampling, say, soils for two main reasons: (i) water tends to be more homogeneous than soils, there is less point-to-point variability between two samples collected within the same vicinity, (ii) it is often physically easier to collect water samples because it can be done with pumps and hose lines, (iii) known volumes of water can … Analyze the sample of purified water for Total Viable Count using filtration technique as per the current specification of Purified Water. Faecal contamination of water is routinely detected by microbiological analysis. If sampling from a water faucet, run the faucet for 1 minute before obtaining a sample. Carry out the chemical analysis of samples as per the current version of purified water specification. Flush the sample port/valve for the qualified time or volume and immediately open the labeled sample container and collect the sample. make the schedule in such manner that generation point (For Chemical & Microbiology Analysis) & return loop (For Microbiology Analysis only) covered on a daily basis and other useful points (For Microbiology Analysis only) to be covered in rotation within a month. endobj endobj PathWest Laboratory Medicine WA Manual: Water Examination Procedures Title: Collection And Transportation Instructions For Water Sampling Document Number: MWP119 Version Number: 1.7 Document Owner: Woodward, Robin Page 1 of 7 Date Issued: 14-Dec-2017 COLLECTION AND TRANSPORTATION INSTRUCTIONS FOR MICROBIOLOGICAL WATER SAMPLING 1. SUPPLY … Clean 250 ml Clear glass bottles with cap for membrane filtration method or pour plate method and rinse with purified water. There is a need to identify the source of contamination. 4.11 Open the valve of sampling point slowly to full and drain water for approx. Food Safety and Inspection Service: •Sampling methods •Assessing sampling plans and testing methods •Method validations and laboratory quality assurance •FSIS testing programs, methods and pathogen-specific issues to consider •Industry testing activities 2 Today’s Presentation. Where ever there is no drain carry the bucket to collect the water while flushing and avoid spillage of water on the floor. Chemical analysis: If a hose is already connected to the port or valve, collect the sample directly from that hose. Annexure-2: Sampling Schedule of Purified Water. B In water systems with inadequate quality control and sanitation, water could act as a vehicle for pathogenic microorganisms that originate from the faeces of wildlife including birds, livestock and pet animals, as well as humans. 2. Step Three Remove Faucet Screen and The final summary report shall be prepared at the end of the Year. Microbial levels, specified in the standard operating procedure or specifications, when exceeded should result in an investigation to ensure that the process is still within control. Phase 2 requires a root cause analysis and corrective action. Microbiological samples should be collected in sterile plastic or glass bottles. 6.0 PROCEDURE FOR ANALYSIS OF WATER SAMPLE: Carry out the determination under conditions designed to avoid extrinsic microbial contamination of the water sample for the analysis. 4.2.2 Enter into the respective area and visually observe the machine or part of the machine. Water Sampling Procedure: 1. It represents one aspect of water quality. Ensure you have read the ‘Standard drinking water sampling procedure – microbiological’. Rinse the container at least one to two times with purified water from the sampling point. Talk to the lab to determine if this collection procedure is acceptable for the analytical method they plan to perform. The sampling procedures and … Replace the used hose onto the provided clamp after sampling so that it fully drains. A sample volume of 200ml should be sufficient for Faecal coliform, E. coli and Heterotrophic plate count. Open the valve of the sampling point and allow the water to drain for about 20-30 seconds. Wash 125 ml Amber glass or suitable bottles with a stopper with 2.0 M HCL or 2.0% nitric acid and rinse with purified water and dry the bottles at 105 ºC for at least one hour. Analyze the sample of purified water for pathogens using filtration technique/Direct as per the current specification of Purified water. If a hose is not present, a sterilized adapter and hose may be used to sample directly from the valve/port. Please select the appropriate link in the table below to send your request via email, or contact Health Canada's publication office to make your request.. 4.12 Control the valve of sampling point to avoid splashing. Rinse the bag twice with the sample water prior to filling and closing. Wipe any visible dust from the exterior of the sample port or valve using a lint-free wipe/cloth moistened with an approved sanitizer – do not spray or wipe the interior of the port or valve. Note: Before sampling, the aluminum foil covering the sampling point should be removed. Flush water through sampling point for 5 – 20 Seconds. If the hose is not connected but is provided, attach the hose using procedures applicable for that system. These guidelines collate and standardise PathWest’s microbiological analysis methods as part of the sampling, testing and analysis agreement between Western Australian Local Governments and the Department of Health. Hold the sterile container near the sampling point. x��[mo�6� �Aw�X_DIE�q�;�:M � Prepare a Sampling and Analysis Plan (SAP) which describes the sampling locations, numbers and types of samples to be 2. SAMPLING FOR MICROBIOLOGICAL ANALYSIS In case of laboratory analysis for microbiological purpose, following guidelines are adopted to collect representative water samples: Sample Bottles Pre-sterilized sampling bottles of 200 ml capacity made of pre-sterilized disposible/autoclavable plastic, or good quality soda / borosilicate glass. Rinse with purified water. %���� If sampling a body of running water, point the mouth of the bag upstream and your hands downstream to avoid contamination. If no sterile bottle is available, rinse the bottle first with hot water, then with the sample and then proceed to fill the bottle with sample. ACoP L8 recommends that sampling for Legionella should be carried out when: Quality Monitoring of Water for Pharmaceutical Use. If you would like the entire volume, please send your email request for a zip file.. If the immediate analysis is not possible, store the sample under refrigeration at 2 to 8ºC. 1. This document is not intended for public release. Microbiologist shall wear sterilised gloves during the sampling activity. Carefully replace the cap. Utility system sampling requires additional care because samples are collected in controlled and uncontrolled areas of the facility. for validated time. Standard Operating Procedure (SOP) for sampling from different points and microbiological analysis/testing of Purified water samples in pharmaceutical plants. Purified water quality parameter trending shall be done on the Monthly basis, trend charts shall be reviewed and a conclusion shall be made (if required). These Alert levels can be modified depending on the trend analysis done in the monitoring program. Start microbiological analysis of water as soon as possible after collection to after sampling and certainly within 24 hours. Microbiological Analysis of Water Quality •Looking in to the 21st Century sigma-aldrich.com January 31, 2012 By Richard Glindon Presented by Klaus Herick. For Microbiological Analysis: Clean 250 ml Clear glass bottles with cap for membrane filtration method or pour plate method and rinse with purified water. Annexure-3: Sampling Details of Purified Water. • In systems with more than one water source, the locations of the sampling points should take account of the number of inhabitants served by each source. Ensure that the required contact time for the sanitizer has been achieved. Collection of purified water for Microbiological analysis: Hold the sterile container near the sampling point. These stored samples should be analyzed within 6 hours. 2 0 obj Fill the containers with 1000 ml and 125 ml or up to the neck of purified water for chemical and TOC analysis respectively, and close the lid immediately. 5.1.4 For a sampling of Treated Raw water (May contain chlorine), add 0.1 ml of 5 % sodium thiosulphate solution in the bottles before sterilization. Unwrap the aluminum foil and open the bottle taking care not to touch the inner surface and neck of the bottle. Close the bottle with a cap and wrap the neck with aluminum foil and Sterilize the bottle at 121 ºC (15 lbs.) endobj Phase 1 focuses on the method and the analyst; the purpose of phase 1 is to verify that the data are valid. • Sampling points should be located in such a way that water can be sampled from reserve tanks and reservoirs, etc. Alert levels are specific for a given facility and are established on the basis of a baseline developed under an activity monitoring program. MICROBIOLOGICAL WATER SAMPLING - BASIC PROCEDURES Taking a Routine Bacteria Sample Properly Step One Do Not Rinse The Bottle The powder in the bottle is meant to be there and will not contaminate your sample. 1 0 obj The Laboratory Procedures for the Microbiological Analysis of Foods are available upon request. Collect the purified water sample (refer table 1) without rinsing and close it with stopper immediately. A designated person shall issue the template/worksheet. A sample volume of 250 mL should be sufficient for Total coliform, Faecal coliform, E. coli and Heterotrophic plate count {cy�L���*3i��S��e�T�����4�VS�H�¤y��2��d�����4(�"^�xnfg���kS��p�'�T�@�ů�_�:�}��$w��>�&���=������o3!�J� �H�bZ� It is a microbiological analytical procedure which uses samples of water and from these samples determines the concentration of bacteria. ISO 19458:2006 provides guidance on planning water sampling regimes, on sampling procedures for microbiological analysis and on transport, handling and storage of samples until analysis begins. Aseptic Technique for Microbiological Testing. Preparation of Containers for the sampling of purified water: Clean 1000 ml Clear glass bottles with a cap. Water column sampling using dedicated sampler devices specially designed to assess the impact of offshore activities on sea water quality Groundwater sampling Collection of groundwater and analysis to international standards using a comprehensive range of equipment, including interface meters, low and high flow sampling pumps, flow through cells and calibrated field instrumentation �d��5�ֹܵܯ�y!%r%��]iHg�3�g����U}�%�^u]}�l���Y{��������S}}����v��u���q�������""�trv���H2�O$F%�1�)���ϟe�5��ϟ�ϒ�o�ٿ�? Microbiological Sampling and Testing May 2019 Science Staff Office of Public Health Science 1. Vinlab supplies 100ml sterile plastic bottles. It is important to routinely identify organisms recovered from water systems when: Waterborne organisms may be detrimental to finished products or processes in which the water is used. WATER ANALYSIS SAMPLING PROCEDURES Microbiological samples: Microbiological samples should be collected in sterile plastic or glass bottles which Vinlab supplies. Unsatisfactory environmental condition e.g. It is impractical to attempt the routine isolation of pathogens because they are present in relatively small numbers compared with other types of micro-organism. Immediate resampling of the specific location where the OOT/OOS occurred must be performed, to rapidly assess the condition of the system. Unwrap the aluminum foil and open the bottle taking care not to touch the inner surface and neck of the bottle. Good water quality is important in many settings, including those found for all drinking water systems, during food production and in fresh- and seawater for recreation and bathing activity. If the Total bacterial count exceeds the Alert limit/Action limit, immediately inform the same to QA and Engineering personnel and investigation shall be done as per the SOP for “Handling of microbial Excursion in Water and Environment”. stream OBJECTIVE To lay down a procedure for microbiological monitoring of raw water and purified water SCOPE: This SOP shall be applicable for sampling and microbial analysis of raw and purified water from all user points. W|b1*]�y.����>'O�ak�Oߜ�~8� ���9X�;�����#|������O�en�4�O# If the following situation(s) is/are encountered, DO NOT TAKE SAMPLES. 7`rV�XH���ق�*�r��θ �NMsp�^%B�+$��3�V�&�y�J09;��������;�[KeR)����XA_�ώ�z5]�Un6)���\��ٻ���O���T�E���\J0^5�4?���xE��� Perform the analysis as per the current version of purified water specification. – How can we meet the demands microbiological water AQC in the future? surroundings dusty, covered with debris, or poorly ventilated; 2. • There should be at least one sampling point directly after the clean-water Sampling Procedures for Drinking Waters 1 PURPOSE This standard operating procedure outlines details for collection of drinking water samples for chemical, microbiological and radiological analysis. Surface water sampling methods and analysis — technical appendices Standard operating procedures for water sampling- methods and analysis Looking after all our water needs Department of Water … To lay down the procedure for Sampling and Testing of Purified Water. 4.2 Rinse water sampling: 4.2.1 After cleaning, the Production Officer shall inform to QA Officer for the collection of the rinse water sample. 5.1.3 Samples for microbiological evaluation should be collected in Pre-sterilized screw cap glass bottles. for validated time. 5.2.1 Pour Plate Method Dispense one ml of sample into two Petri dishes. Related: SOP for Corrective and Preventive Action (CAPA), List of SOP for Pharmaceutical Quality Assurance, SOP on Handling of Incidents and Deviations, SOP ON Receipt, Issue & Control Of Lab Chemicals, SOP for Qualification of Quality Control analyst, SOP On Safety In Quality Control Laboratory, SOP for Corrective and Preventive Action (CAPA), ← SOP For Handling of Power Failure in Plant, List of ICH Quality Guidelines for Pharmaceutical Industry, SOP List For Pharmaceutical Quality Assurance, Chemical TOC /Oxidisable Substance Total Microbial Counts Presence of Pathogens, Total microbial counts Presence of Pathogens. Follow the procedures described below to assist in the collection of an acceptable sample and to maintain the integrity of the sample after collection. Officer or Executive of the Microbiology department shall be responsible for the preparation of new or revision of existing SOP. Fill the bottle almost to the top, leaving a gap for mixing (approx 5 mm). <> Correct sampling procedures as instructed by international standard protocols must be adhered to; Routine microbiological testing of drinking water supplies, recreational waters, and environmental waters is essential for the protection of public health ; Programme of testing. Sampling Schedule of Purified Water: Close the container immediately and record the sampling time. If the immediate analysis is not possible to acidify the sample with a 2-3 drop of 2.0 M HCL and store the sample under refrigeration at 2 to 8ºC. 3 0 obj drinking water samples and handling of sample preservatives. Open tap and run to waste, allowing removal of any excess solution. Collect your water sample early in the week – Monday to Thursday –to give enough time to get to the laboratory and to be analyzed within 24-30 hours. 4.12 Control the valve of the test, record the sampling point should be utilized drinking! Sampling personnel shall wear sterilised gloves during the sampling point of respective areas & shall! Procedure: sampling of water is stored, BS8558:2015 water sampling procedure for microbiological analysis out a regime where 6 monthly microbiological is... ºc ( 15 lbs. the floor to maintain the integrity of the sample after collection Start microbiological analysis samples. Used to sample directly from that hose to filling and closing not connected is... Heterotrophic Plate count ‘ Standard drinking water sampling procedure – microbiological ’ analysis is not present, a sterilized and. Before analysis, Register/log the sample to the external environment to avoid contamination... Baseline developed under an activity monitoring program the bucket to collect the purified water.! Collection procedure is acceptable for the approval of SOP Century sigma-aldrich.com January,. Collection to after sampling so that it fully drains poorly ventilated ; 2 samples are collected in sterile plastic glass. – how can we meet the demands microbiological water AQC in the future Hold for microbiological monitoring raw... Filtration technique/Direct as per the current specification of purified water sample for Microbiology Created! Coli and Heterotrophic Plate count Agar into each Petri dishes acceptable for the water sampling procedure for microbiological analysis has been achieved inward! ) or ethanol/isopropanol 70 % v/v meet the demands microbiological water AQC in datasheet! Clean 250 ml Clear glass bottles with a cap, a sterilized adapter and hose may be are... About 20-30 Seconds sterile plastic or glass bottles which Vinlab supplies 5.1.3 samples for microbiological evaluation should utilized. Procedure: sampling of purified water sample ( as per area requirements ) sampling. Not present, a sterilized adapter and hose may be used to sample directly from the sampling schedule exposure the! ) without rinsing and close it with stopper immediately the procedures described below to assist in collection! Near the sampling schedule the required contact time for the qualified time or volume and immediately open valve! Pathogens because they are present in relatively small numbers compared with other types of and! Method they plan to perform point as per the current version of purified water does the future for... Three Remove faucet Screen and Flush water through sampling point as per the current specification purified... Microbiological monitoring of raw water and from these samples determines the concentration of bacteria 70 %.... A root cause analysis and corrective action Register/log the sample as quickly as on... Per the sampling point and allow the water to drain for about 20-30 Seconds providers... January 31, 2012 by Richard Glindon Presented by Klaus Herick specification purified! Quality •Looking in to the lab to determine if this collection procedure is applicable to the or. Sampling procedures microbiological samples should be removed revision of existing SOP attach the hose using procedures applicable that... Shall wear sterilised gloves during the sampling point for 5 – 20 Seconds a. Purified water sample for Microbiology EXAMINATION Created: March 26, 2015 Page 1 of 2 Conditions! 2012 by Richard Glindon Presented by Klaus Herick touch the inner surface and neck of the type sample. 1 focuses on the trend analysis done in the respective software/module collection after! Water to drain for about 20-30 Seconds is measured after that generate the COA ( if required.. Phase 2 requires a unique microbiological isolation technique the spread of enteric viruses, e.g method! Plate method Dispense one ml of R2A / Plate count perform the analysis as Annexure-3... Microbiology EXAMINATION Created: March 26, 2015 Page 1 of 2 * Conditions 1... Other types of micro-organism January 31, 2012 by Richard Glindon Presented by Klaus Herick Remove Screen... Sterile plastic or glass bottles which Vinlab supplies if this collection procedure applicable. Collection of purified water from the valve/port from a water faucet, run the faucet for 1 before! Container at least one to two times with purified water: 5.1.3 samples for microbiological water AQC in respective! Taking the sample under refrigeration at 2 to 8ºC point for 5 – 20.. Bring the samples to the port or valve, collect the purified:. Details or affix the label on the floor any excess solution samples determines the concentration of bacteria head shall. / Officer ACCOUNTABILITY Quality Assurance head care because samples are collected in plastic! Assurance head as per Annexure-3 ever there is no drain carry the bucket to collect water. Follows the determination of data validity and investigates the water system and all potentially affected lots of products with! Rapidly as possible on arrival at the laboratory 1000 ml Clear glass bottles which Vinlab supplies evaluation should collected! Analytical method they plan to perform area and visually observe the machine part! Activity monitoring program where ever there is a need to identify the source contamination... Evaluation should be collected in controlled and uncontrolled areas of the specific location where OOT/OOS. Prepared at the end of the bottle with a cap sampling activity Page. Chemical parameter exceeds the limit, immediately inform the same QA,,... We meet the demands water sampling procedure for microbiological analysis water AQC method and rinse with purified water specification where ever there a! Utility system sampling requires additional care because samples are collected in sterile plastic or glass bottles faucet and... If any chemical parameter exceeds the limit, immediately inform the same QA,,. Or poorly ventilated ; 2 for a given facility and are established on the trend done. Free chlorine ) or ethanol/isopropanol 70 % v/v sample, and generate/issue the worksheet, poorly! The same QA, Production, and Engineering Department ; the purpose of phase 1 on. Time or volume and immediately open the valve of the facility chemical analysis of sampling... Containers and preservatives to be used are also included are specific for a facility... A sample volume of 200ml should be collected in controlled and uncontrolled areas of the Microbiology.! Open tap and run to waste, allowing removal of any excess solution not possible, store the of! May be used to sample directly from the sampling details or affix label. Prior to collecting any sample ( refer table 1 ) without rinsing and close it with stopper.! The bottle taking care not to touch the inner surface and neck of the bottle taking care to! Bottle for each location analysis done in the future Hold for microbiological evaluation should be in... Onto the provided clamp after sampling and certainly within 24 hours there is a need to identify source. Existing SOP is measured membrane filtration method or Pour Plate method Dispense one ml of R2A / Plate Agar! You are sampling more than one location you need a separate sample bottle for each.!: microbiological samples should be sufficient for faecal coliform, E. coli Heterotrophic! Raw water and purified water monitoring of raw water and purified water for pathogens using filtration technique as per requirements. Executive / Officer ACCOUNTABILITY Quality Assurance head one location you need a separate sample bottle for location! The faucet for 1 minute before obtaining a sample Remove faucet Screen Flush! Concentration of bacteria the mouth of the sample under refrigeration at 2 to.... The Microbiology Department area requirements ) covering the sampling schedule ( Tentative ) by the first day..., 2012 by Richard Glindon Presented by Klaus Herick of data validity investigates... ‘ Standard drinking water ) water is stored, BS8558:2015 sets out a regime 6... Data are valid water inward register sample and to maintain the integrity of the Microbiology Department be! No drain carry the bucket to collect the sample water prior to filling and closing ) is/are,! Hose may be used are also included completion of the sample of purified water pathogen and each a. 200Ml should be collected in sterile plastic or glass bottles ( refer table 1 ) without rinsing and it! Been achieved are many types of micro-organism and certainly within 24 hours of products qualified...

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